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目的:寻求适合大鼠骨髓间充质干细胞实时监测培养的方法。方法:从SD大鼠(7天大)骨髓中提取间充质干细胞,将其诱导分化为成骨细胞进行分化能力的检测。并将间充质干细胞置于自主研发的可持续灌注培养系统中,考察其对间充质干细胞的培养与实时监测。结果:成功提取骨髓间充质干细胞;诱导分化染色之后,可观察到大量钙结节,证明其具有良好的成骨化能力;间充质干细胞在可持续灌注细胞培养系统中培养24小时,可以达到与CO2培养箱相同的培养效果。结论:自主研发的可持续灌注培养系统可用于大鼠骨髓间充质干细胞的实时连续监测培养。
Objective: To find a suitable method for real-time monitoring and culture of rat bone marrow mesenchymal stem cells. Methods: Mesenchymal stem cells (MSCs) were extracted from the bone marrow of SD rats (7 days old) and then induced to differentiate into osteoblasts for differentiation assay. Mesenchymal stem cells were placed in a self-developed sustainable perfusion culture system to investigate the culture and real-time monitoring of mesenchymal stem cells. RESULTS: Bone marrow-derived mesenchymal stem cells were successfully extracted. After induction of differentiation staining, a large number of calcium nodules were observed and proved to have good osteogenic ability. Mesenchymal stem cells cultured in a sustainable perfusion cell culture system for 24 hours, Achieve the same culture effect as a CO2 incubator. Conclusion: The self-developed sustainable perfusion culture system can be used for real-time continuous monitoring of rat bone marrow mesenchymal stem cells.