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Objective:The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L.leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate stones.Methods:This survey was carried out using hydroalcoholic extract (E.FA) and infusion (I.FA) of A.unedo leaves.The quantification of phenolic compounds,flavonoids,flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultraperformance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry.Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and by the ferric reducing/antioxidant power (FRAP) assay.Litholytic activity of E.FA and I.FA was studied using a special model that resembles circuitry of the urinary system.Results:E.FA showed greater antioxidant efficacy than I.FA (P ＜ 0.05).Its higher efficiency was shown via the values of median inhibitory concentration,which was close to (76.14 ± 0.91) μg/mL for E.FA versus (202.64±5.77) μg/mL for I.FA using the DPPH method,and (53.77±0.81) μg/mL for E.FA versus (236.86 ± 31.90) μg/mL for I.FA,using FRAP method.I.FA exhibited significantly higher litholytic activity compared to E.FA (P＜ 0.05),with dissolution values of 31.03％ ± 0.63％ versus 14.55％ ± 0.65％,respectively.Conclusion:Overall,the results suggest that the A.unedo is rich in bioactive compounds,and possesses antioxidant and litholitic abilities that are worthy of further study.