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[目的]为益生菌及有机硒在鳗鲡健康养殖中的应用提供理论依据。[方法]将长孢娄徳酵母、乳明串珠菌、枯草芽孢杆组成复合益生菌,并通过调整富硒长孢娄徳酵母的含量在复合益生菌中添加不同浓度的有机硒后投喂日本鳗鲡,探讨益生菌及有机硒对鳗鲡生长性能、抗氧化水平和免疫力的影响。[结果]制备的富硒酵母(Se-Y1)的生物量为6.94 g/L,菌体硒含量为1 446.90μg/g,其中有机硒含量为1 377.88μg/g,有机硒转化率为95.24%。投喂45 d后,益生菌可以显著提高幼鳗的生长性能以及肝脏SOD、CAT活性,并降低饵料系数以及肝脏组织的MDA、NO含量,还可以提高血清溶菌酶活力。投喂125 d后,上述指标差异不显著。0.4 mg/kg有机硒没有进一步提高益生菌对鳗鲡的有益作用,而0.8 mg/kg有机硒则抑制了益生菌的作用。[结论]长孢娄徳酵母、乳明串珠菌、枯草芽孢杆菌在日本鳗鲡幼鳗生长早期可以提高其生长性能、抗氧化水平和溶菌酶活性。
[Objective] The research aimed to provide the theoretical basis for the application of probiotics and organic selenium in the healthy cultivation of eel. [Method] The compound Probiotics were composed of L. longifolia, L. margarita and B. subtilis, and fed into Japan after adding different concentrations of organic selenium by adjusting the content of L. sep The effects of probiotics and organic selenium on the growth, antioxidation and immunity of eel Anguilla. [Result] The biomass of selenium-enriched yeast (Se-Y1) was 6.94 g / L, the content of selenium was 1 446.90 μg / g, the content of organic selenium was 1 377.88 μg / g and the conversion of organic selenium was 95.24 %. After 45 days of feeding, probiotics can significantly improve the growth performance of eel and liver SOD, CAT activity, and reduce the feed conversion coefficient and liver tissue MDA, NO content, but also can improve the activity of serum lysozyme. After 125 days of feeding, there was no significant difference between the above indexes. 0.4 mg / kg organic selenium did not further improve the beneficial effect of probiotics on eel, while 0.8 mg / kg organic selenium inhibited the effect of probiotics. [Conclusion] The growth, antioxidation and lysozyme activities of L. elongatus, L. margarita and B. subtilis in the early stage of growth of Japanese eel Anguilla.