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丙型肝炎是由丙型肝炎病毒(HCV)引起的一种传染病,主要通过输血等肠道外途径传播.为预防经血液传播丙型肝炎,我们于1996年对187例职业献血员进行抗—HCV及谷丙转氨酶(ALT)的血清学检测,并设立健康对照组进行比较分析,现报告如下:1 材料与方法1.1 检测对象 献血员187例,其中男73例,女114例,年龄25~50岁.献血1年以下142例.1~5年30例,超过5年15例,乙型肝炎表面抗原(HBsAg)均阴性,其他体格检查无异常.选152例健康体检者,男65例,女87例,年龄25~48岁,无输血及使用血制品史.肝功能正常,HBsAg均阴性,ALT<25单位(卡门氏单位)作为对照组.1.2 方法 抗-HCV测定采用上海科华生物技术有限公司的抗—HCV试剂盒,EIA法.按说明书操作.
Hepatitis C is an infectious disease caused by the hepatitis C virus (HCV) and is transmitted mainly by the parenteral route such as blood transfusion. In order to prevent the spread of hepatitis C through bloodstream, in 1996, we conducted 187 professional blood donors against anti- HCV and alanine aminotransferase (ALT) serological detection, and the establishment of a healthy control group for comparative analysis, are as follows: 1 Materials and methods 1.1 Detection of blood donation 187 cases, 73 males and 114 females, aged 25 ~ 50 years old.And donate blood for less than one year in 142 cases.Among them, there were 30 cases in 1 ~ 5 years and 15 cases more than 5 years.Healthy hepatitis B surface antigen (HBsAg) , 87 females, aged 25 to 48 years old, no history of blood transfusion and the use of blood products.Her liver function was normal, HBsAg were negative, ALT <25 units (Carmen unit) as a control group.2.2 Methods Anti-HCV assay using Shanghai Kehua Biotechnology Co., Ltd. anti-HCV kit, EIA method. Follow the instructions.