论文部分内容阅读
目的 :探讨大鼠补充一定剂量牛磺酸及微量营养素后 ,能否通过影响视感受器或视中枢NO合成酶 (NOS)表达及第二信使 (cGMP)合成 ,影响视觉信号传导。方法 :Wistar大鼠随机分为三组 ,即对照组 (正常饲料组 )、实验 1组 (5倍需要量组 )和实验 2组 (10倍需要量组 ) ,喂养 3周后 ,每组动物再随机分为光照组和暗适应组 (平均照度为3 0 3LX) ,以正常饲料喂养 72h ,大鼠活杀取样 ,以放射免疫方法分析cGMP含量 ,NDP组化染色检测NOS表达。结果 :在暗适应条件下 ,视网膜光感受体细胞NOS表达 ,视皮层、外侧膝状体、顶盖前区或海马cGMP含量增加 ,而在明适应状态下 ,NDP组化染色浅淡 ,牛磺酸及微量营养素的干预则能明显增强暗适应条件下NOS的着色反应 ,增加光照及暗适应条件下上述脑功能区cGMP含量。结论 :不同的光适应状态下 ,NOS、cGMP在视觉感受器及视中枢的分布、表达及含量是不同的 ,牛磺酸及微量营养素能明显增加暗适应条件下NO合成及cGMP含量
OBJECTIVE: To investigate whether optic nerve signal transduction can be influenced by affecting the expression of visual receptor or central NO synthase (NOS) and the synthesis of second messenger (cGMP) after taurine and micronutrient supplementation in rats. Methods: Wistar rats were randomly divided into three groups: control group (normal feed group), experimental group 1 (5 times required dose group) and experimental group 2 (10 times required dose group). After feeding for 3 weeks, Then the rats were randomly divided into light group and dark adaptation group (average illuminance was 303LX). The rats were fed with normal diet for 72h. The rats were killed by live sampling and radioimmunoassay was used to analyze the content of cGMP. NOS expression was detected by NDP histochemical staining. RESULTS: Under dark adaptation, NOS expression in retina photoreceptor cells increased cGMP levels in the visual cortex, lateral geniculate body, anterior prepuce or hippocampus, whereas in the bright adaptation state, NDP staining was weak, while taurine The intervention of acid and micronutrient can obviously enhance the color reaction of NOS under dark adaptation conditions, and increase the content of cGMP in brain function zone under light and dark adaptation conditions. CONCLUSION: The distribution, expression and content of NOS and cGMP are different in the photoreceptors and central visual cords under different light adaptation states. Taurine and micronutrient can significantly increase NO synthesis and cGMP content under dark adaptation