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目的:按6.82 mg/(kg·bw)大鼠单剂量口服臭豆碱,用反相离子对高效液相色谱方法,测定大鼠肾组织中不同时间点臭豆碱的浓度。方法:高效液相色谱法,色谱柱为大连产HYP-ODS柱(200 mm×4.6 mm,5μm),柱温30℃;流动相由水相-异丙醇-乙腈(150∶15∶20体积比)组成。水相部分每1000 mL中含SDS 0.5g,三乙胺3.5 mL,85%磷酸2 mL(用三乙胺调至PH 3.0);流速1.0 mL·min-1紫外检测波长309 nm。结果:经DASver1.0药代动力学软件处理数据,臭豆碱在肾组织符合一室模型,其动力学参数为:Tmax(60)min,Cmax(13747.80)μg/L,T1/2(113.34)min,T1/2ka(16.19)min,AUC(3033978)μg/L·min,Vd/F(16.193 L/kg),CL/F(0.005)L/min/kg。结论:臭豆碱灌服后,可迅速分布到肾脏,具有消除快、蓄积时间短的特点。
OBJECTIVE: To orally take stanni base at a single dose of 6.82 mg / (kg · bw), and to determine the concentration of stanni base in rat kidney at different time points by reversed-phase ion-pair high performance liquid chromatography. Methods: High performance liquid chromatography (HPLC) was performed on a HYP-ODS column (200 mm × 4.6 mm, 5 μm) with a column temperature of 30 ℃. The mobile phase consisted of a mixture of water and isopropanol- Than) composition. The aqueous phase contained 0.5g of SDS per 1000mL, 3.5mL of triethylamine and 2mL of 85% phosphoric acid (adjusted to pH 3.0 with triethylamine). The flow rate was 1.0 mL · min-1. UV detection wavelength was 309 nm. Results: According to the data of pharmacokinetics software DASver1.0, the kinetic parameters of stachydrine in renal tissue were Tmax (60) min, Cmax (13747.80) μg / L, T1 / 2 (113.34 ), T1 / 2ka (16.19) min, AUC (3033978) μg / L · min, Vd / F (16.193 L / kg) and CL / F (0.005) L / min / kg. Conclusion: After stained with metronidazole, it can be quickly distributed to the kidneys and has the characteristics of quick elimination and short accumulation time.