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本文介绍了一种灵敏测定大白鼠皮下徽透析液中葡萄糖的液相色谱-电化学(LCEC)分析方法.本法用柱后葡萄糖氧化酶反应器将色谱分析柱中分离出的葡萄糖转化成过氧化氢,再用过氧化物酶电极检测生成的过氧化氢.本法中的过氧化物酶电极是将含有过氧化物酶的氧化还原聚合物涂覆在破碳电极表面制成的,可于+100 mV(υs.Ag/AgCl)测定过氧化氢的还原电流.与常规的柱后检测过氧化氢的铂电极相比,过氧化物酶电极显著提高了灵敏度、检测限和检测稳定性.
This article presents a liquid chromatography-electrochemical (LCEC) method for the sensitive determination of glucose in the dialysate of rat subcutaneous emblem. This method uses a post-column glucose oxidase reactor to convert glucose isolated from a chromatographic column Hydrogen peroxide, and then use the peroxidase electrode to detect the generated hydrogen peroxide peroxidase electrode in this law is the peroxidase-containing redox polymer coated on the surface of carbon-broken electrodes made of Determination of hydrogen peroxide reduction current at +100 mV (υs.Ag / AgCl) Peroxidase electrode significantly improves sensitivity, detection limit and detection stability compared to conventional post-column detection of hydrogen peroxide in platinum electrode .