论文部分内容阅读
目的探讨雌性小鼠孕期给予丰富环境刺激对子代新生小鼠空间学习记忆能力的影响。方法选择SPF级C57BL/6受孕雌鼠24只随机分为丰富环境组和标准环境组,在整个孕期分别给予丰富环境、普通环境的刺激直至分娩为止。两组小鼠出生后均移至普通环境饲养,在出生后的1个月及2个月时分别对子代小鼠进行水迷宫行为学检测、海马区新生神经元的免疫荧光检测。结果在1月龄的子代小鼠中,定位航行实验显示丰富环境组比普通环境组小鼠的平均逃避潜伏期时间明显缩短(P<0.05),而空间探索实验显示丰富环境组平台象限游泳距离百分比和穿台次数均高于普通环境组(P<0.05)。在海马CA1区和齿状回,丰富环境组神经元前体细胞标记物微管相关蛋白Doublecortin(DCX)阳性神经细胞数目较普通环境组明显增多(P<0.05)。而在2月龄的子代小鼠检测中,两组小鼠在上述指标均无明显统计学差异。结论在母鼠妊娠期间给予丰富环境刺激可以一过性提高子代小鼠的学习记忆能力以及神经发生发育。
Objective To investigate the effect of rich environmental stimuli during pregnancy on spatial learning and memory abilities of offspring neonatal mice. Methods Twenty-four female SPF C57BL / 6 pregnant women were randomly divided into rich environment group and standard environment group, which were given rich environment and normal environment stimulation respectively until the childbirth. After the birth of both groups, the mice in both groups were moved to normal environment for feeding. At 1 month and 2 months after birth, water maze behavior test and immunofluorescence detection of neonatal neurons were performed respectively in offspring mice. Results In the 1-month-old offspring mice, the navigation experiment showed that the average evacuation latency time was significantly shorter in the rich environment group than in the normal environment group (P <0.05), while the space exploration experiment showed that the enriched environment group platform quadrant swimming distance The percentage and the number of wearing the platform were higher than the normal environment group (P <0.05). In the hippocampal CA1 and dentate gyrus, the number of doublecortin (DCX) positive neuronal precursors of neuronal precursor cells in the enriched enviroment group was significantly higher than that in the normal enviroment group (P <0.05). In the 2-month-old offspring mice test, the two groups of mice in the above indicators were no significant difference. Conclusion Abundant environmental stimulation during pregnancy in maternal mice can transiently improve learning and memory abilities and neurogenesis in offspring mice.