论文部分内容阅读
目的探讨雌性和雄性成年大鼠脑缺血再灌注海马齿状回颗粒下层(subgranular zone,SGZ)、侧脑室室下层(subventricular zone,SVZ)和梗死皮质周边神经干细胞(neural stemcell,NSC)增殖的差异性。方法雌性和雄性成年大鼠各16只,制作线栓法大脑中动脉闭塞2h后再灌注模型,用免疫组织化学方法动态检测海马齿状回SGZ、SVZ和梗死皮质周边5-溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdU)标记的阳性细胞数的变化,并采用HPIAS图像分析系统进行对比分析。结果雄性大鼠SGZ、SVZ及梗死周边区域Br-dU阳性细胞在缺血再灌注3d开始增加,7d达到高峰,11d后开始下降,18d进一步减少。而雌性大鼠则是3d开始增加,7d明显增加,11d达到高峰,18d才开始减少,且各时间点雌性大鼠的BrdU阳性细胞数均明显高于雄性大鼠。结论脑缺血再灌注雌雄成年大鼠均有NSC增殖,雌性大鼠NSC增殖较雄性大鼠更为明显。
Objective To investigate the proliferation of subgranular zone (SGZ), subventricular zone (SVZ) and neural stem cell (NSC) in the hippocampus of adult and male adult rats after cerebral ischemia / reperfusion. difference. Methods Twenty-six adult female and male adult rats were used to establish a reperfusion model of middle cerebral artery occlusion 2 h after thread occlusion. Immunohistochemistry was used to detect the SGZ, SVZ of hippocampus and 5-bromodeoxyuridine nucleus around the infarct cortex (Bromodeoxyuridine, BrdU) labeled positive cells, and analyzed by HPIAS image analysis system. Results BrdU positive cells in SGZ, SVZ and infarct peripheral area of male rats began to increase at 3d after ischemia-reperfusion, peaked at 7d, decreased at 11d and decreased at 18d. The female rats began to increase 3d, 7d significantly increased, peaked on the 11d, 18d began to decrease, and the number of BrdU positive cells in each time point were significantly higher than male rats. Conclusion Both male and female adult rats with cerebral ischemia and reperfusion have NSC proliferation. The proliferation of NSC in female rats is more obvious than that in male rats.