论文部分内容阅读
应用杂交瘤技术 ,建立了 5株分泌抗弓形虫单克隆抗体的杂交瘤细胞。其中 1E9和 4C10 2株分泌的 Mc Ab效价达 1∶ 12 80 0。该 2株单克隆抗体均属 Ig G1亚类 ,其识别的弓形虫抗原分别为 P2 2、P2 8。1E9和 4C10 Mc Ab混合后与兔抗弓形虫抗体联合 ,进行双抗体夹心 EL ISA测定弓形虫的敏感性分别为 12 .5和 2 5个虫体 / m l。检测 146份有不良孕产史妇女血清 ,11份弓形虫循环抗原 ( CAg)阳性 ( 7.5 3% )。检测 11份自然流产物 ,1份 CAg阳性 ,该流产物转种小鼠后证实确含有弓形虫。 92份育龄妇女血清 1份弓形虫 CAg阳性 ( 1.0 9% )。有不良孕产史的妇女血清弓形虫 CAg阳性率明显高于育龄妇女血清阳性率 ( P<0 .0 5 )。弓形虫经 1E9、4C10 Mc Ab处理后对小鼠腹腔巨噬细胞攻击 ,感染率分别为 7.8%和 7.5 % ,而对照组感染率为 14.5 % ,证明该 2株单克隆抗体对弓形虫有明显的抑制作用
Five hybridoma cells secreting monoclonal antibodies against Toxoplasma gondii were established by using hybridoma technology. Among them, 1E9 and 4C10 2 strains secreted McAb titers of 1: 1280 0. The two monoclonal antibodies belong to the subclass Ig G1, and the identified Toxoplasma gondii antigens were P2 2, P2 8.1E9 and 4C10 Mc Ab, respectively, and then combined with anti-Toxoplasma gondii antibody to detect the arch shape by double-antibody sandwich ELISA Insect sensitivity was 12.5 and 25 worms / ml, respectively. A total of 146 sera from women with adverse pregnancy history were detected, and 11 were positive for Toxoplasma gondii (CAg) (7.5 3%). 11 natural products were detected and 1 CAg was positive. The Toxoplasma gondii was confirmed after the mice were transplanted into mice. A total of 92 Toxoplasma gondii (92%) women of childbearing age were positive (1.09%). The positive rate of serum CAg of Toxoplasma gondii in women with history of adverse pregnancy was significantly higher than that in women of childbearing age (P <0.05). The Toxoplasma gondii challenged mouse peritoneal macrophages by 1E9 and 4C10 McAbs, and the infection rates were 7.8% and 7.5% respectively, while the infection rate in the control group was 14.5%. The results showed that the two monoclonal antibodies showed obvious effects on Toxoplasma gondii Inhibition