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目的探讨食管癌变过程中细胞增生调控的分子学机制。方法利用免疫组织化学技术研究66例原发性食管癌和癌旁正常及各级病变组织中p-Tyr蛋白和C-erbB-2表达状况,进行检测分析。结果p-Tyr蛋白和C-erbB-2从正常食管上皮到基底细胞过度增生、不典型性增生和鳞癌,免疫阳性表达率分别为18%、46%、58%、61%和14%、60%、37%和89%;从高分化到中分化及低分化鳞癌,阳性率分别为82%、64%、44%和94%、100%和75%;食管癌组织中C-erbB-2免疫反应阳性病灶中p-Tyr阳性率为64%(21/33),C-erbB-2免疫反应阴性病灶中p-Tyr阴性率为50%(2/4),但p-Tyr和C-erbB-2相关性分析显示二者无相关性;从形态学正常的食管上皮到BCH、DYS、SCC,p-Tyr和C-erbB-2局灶型分布的百分率分别为46%、66%、36%、37%和50%、67%、48%和35%,弥漫型分布的百分率分别为44%、34%、64%、53%和50%、33%、52%和65%。结论p-Tyr蛋白和C-erbB-2表达变化发生于食管癌变早期阶段,二者可能是参与食管癌变发生过程的重要分子学事件;p-Tyr蛋白可能是食管癌预后的重要生物学指标之一,酪氨酸蛋白的磷酸化可能是食管上皮癌变多阶段演进过程中细胞增生调控相关的重要信号转导形式之一。
Objective To investigate the molecular mechanism of cell proliferation regulation in esophageal carcinogenesis. Methods The expression of p-Tyr protein and C-erbB-2 in 66 cases of primary esophageal carcinoma, paracancerous normal tissues and lesions were studied by immunohistochemistry. Results The positive rate of p-Tyr and C-erbB-2 from normal esophageal epithelium to basal cell hyperplasia, atypical hyperplasia, and squamous cell carcinoma were 18%, 46%, 58%, 61%, and 14%, respectively. 60%, 37%, and 89%; from well-differentiated to moderately differentiated and poorly differentiated squamous cell carcinoma, the positive rates were 82%, 64%, 44%, and 94%, 100%, and 75%, respectively; C-erbB in esophageal cancer tissue The positive rate of p-Tyr was 64% (21/33) in positive lesions of -2 immunoreaction, and 50% (2/4) in negative lesions of C-erbB-2 immunoreactivity, but p-Tyr and C-erbB-2 correlation analysis showed no correlation between the two; the percentage of focal type distribution from normal oesophageal epithelium to BCH, DYS, SCC, p-Tyr and C-erbB-2 was 46%, 66 respectively. %, 36%, 37% and 50%, 67%, 48% and 35%, the percentage of diffuse distribution was 44%, 34%, 64%, 53% and 50%, 33%, 52% and 65%, respectively . Conclusion The expression changes of p-Tyr protein and C-erbB-2 occur in the early stage of esophageal carcinogenesis. The two may be important molecular events involved in the process of esophageal carcinogenesis; p-Tyr protein may be an important biological indicator of the prognosis of esophageal cancer. First, the phosphorylation of tyrosine protein may be one of the important signal transduction forms related to cell proliferation regulation during the multi-stage evolution of esophageal carcinoma.