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目的 探讨人类非小细胞肺癌组织中p16基因缺失及突变情况及其与临床病理的关系。 方法 应用控制模板DNA量的银染PCR-SSCP技术检测40例非小细胞肺癌手术切除标本中p16基因第2外显子。结果 40例中有13例发生纯合缺失，3例发生突变，缺失及突变率为40%。其缺失及突变率与肺癌的临床病理分期有密切关系（Ｐ<0.05）。 结论 p16基因的缺失及突变可能在非小细胞肺癌的发生、发展及转移中起重要作用。
Objective To investigate the deletion and mutation of p16 gene in human non-small cell lung cancer (NSCLC) and its relationship with clinical pathology. Methods Silver staining PCR-SSCP technique was used to detect the exon 2 of p16 gene in 40 specimens of non-small cell lung cancer. Results Homozygous deletion was found in 13 of 40 cases, mutation occurred in 3 cases, and deletion and mutation rate was 40%. The deletion and mutation rate were closely related to the clinical pathological stage of lung cancer (P<0.05). Conclusion The deletion and mutation of p16 gene may play an important role in the occurrence, development and metastasis of non-small cell lung cancer.