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目的研究新型光敏剂铝酞菁Ｄ（ＡｌＰＣ－Ｄ）单用及与黄夹甙或维拉帕米合用对体外培养肿瘤细胞的光动力治疗作用。方法细胞经药物作用２ｈ后，用红光辐照一定时间，继续培养２４ｈ，台盼蓝排染法测定药物对肿瘤细胞的抑制率。结果２．５～１０．０μｇ／ｍｌＡｌＰＣ－Ｄ配以５．４或１０．８Ｊ／ｃｍ２的红光射照，能显著杀伤Ｋ５６２和ＳＧＣ７９０１细胞，并有剂量依赖性。０．００１和０．０１μｇ／ｍｌ黄夹甙或单用无效剂量的维拉帕米（５μｇ／ｍｌ）与ＡｌＰＣ－Ｄ合用，能增强后者对Ｋ５６２细胞的光动力作用。结论ＡｌＰＣ－Ｄ对体外培养的肿瘤细胞具有光动力治疗作用，黄夹甙和维拉帕米能增强其作用。
Objective To study the photodynamic therapy effects of a novel photosensitizer Al (P <0.01), AlPC-D, alone or in combination with lutein or verapamil on cultured tumor cells in vitro. Methods The cells were treated with drugs for 2 h, irradiated with red light for a certain period of time, and cultured for 24 h. Trypan blue dyeing method was used to determine the inhibitory rate of the drug on tumor cells. Results 2.5～10.0μg/ml AlPC-D combined with 5.4 or 10.8J/cm2 red light irradiation can significantly kill K562 and SGC7901 cells in a dose-dependent manner. The combination of 0.001 and 0.01 μg/ml of yellow pincers or single dose of verapamil (5 μg/ml) with AlPC-D can enhance the photodynamic effect of the latter on K562 cells. Conclusion AlPC-D has a photodynamic therapy effect on tumor cells cultured in vitro. The effects of Alfalfa and Verapamil can be enhanced by AlPC-D.