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目的 :探讨LFA 1与抗CD3mAb共刺激对狼疮肾炎 (LN)患者外周血单个核细胞 (PBMC)增殖与免疫球蛋白 (IgG)合成的影响。方法 :分离LN患者及健康人 (正常对照 )的PBMC ,采用Ficoll梯度密度离心法。细胞增殖实验采用3 H TdR掺入法。IgG测定采用ELISA法。结果 :在抗CD3mAb诱导下 ,2 9例LN非活动期和活动期PBMC中 ,3 H TdR的掺入量和IgG的合成均增加 ,且活动期的增殖强于非活动期 (P <0 .0 1) ;而单纯用抗CD3mAb对 12例正常对照的PBMC没有影响 (P >0 .0 5 )。抗CD3mAb与抗LFA 1mAb共刺激 ,均可增加LN患者 (活动期和非活动期 )及正常对照PBMC3 H TdR掺入量和IgG合成 ,其中活动期增殖效应强于非活动期 (P <0 .0 1) ,后者又强于正常对照 (P <0 .0 1)。抗LFA 1中和抗体的加入 ,可明显抑制LFA 1诱导的共刺激效应 (P <0 .0 1)。结论 :LFA 1促进LN患者PBMC增殖和IgG合成 ,可能是其参与LN发病的机制之一
Objective: To investigate the effects of costimulation of LFA 1 and anti-CD3 mAb on the proliferation and immunoglobulin (IgG) synthesis of peripheral blood mononuclear cells (PBMCs) in patients with lupus nephritis (LN). METHODS: PBMCs from patients with LN and healthy controls (normal controls) were isolated and analyzed by Ficoll gradient density centrifugation. Cell proliferation experiments using 3 H TdR incorporation. IgG assay using ELISA method. RESULTS: In the 29 non-active and active PBMCs induced by anti-CD3 mAb, the amount of 3 H TdR incorporation and IgG synthesis increased, and the proliferative activity in active phase was stronger than that in non-active phase (P <0. 0 1). However, anti-CD3 mAb alone had no effect on PBMC of 12 normal controls (P> 0.05). Both anti-CD3 mAb and anti-LFA 1 mAb co-stimulated increased the amount of 3H-TdR incorporation and IgG synthesis in PBMCs of LN patients (active and inactive) and normal controls, with the proliferative effect of active phase being stronger than that of non-active phase (P <0. 0 1), which is stronger than the normal control (P <0.01). The addition of anti-LFA 1 neutralizing antibody significantly inhibited the LFA 1 -induced co-stimulatory effect (P <0.01). CONCLUSION: LFA 1 can promote PBMC proliferation and IgG synthesis in patients with LN, which may be one of the mechanisms involved in the pathogenesis of LN