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Transgenic ruminants are a valuable resource for both animal breeding and biomedical research.The development of transgenic breeding is proceeding slowly,because it suffers from low efficiency of gene transfer and possible safety problems from uncontrolled random integration.However,new breeding methods combined with genome editing and somatic cell nuclear transfer or microinjection can offer an economic and efficient way to produce gene-edited ruminants,which can serve as bioreactors or have improved disease resistance,animal welfare and product quality.Recent advances in precise genome editing technologies,especially clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 nucleases,are enabling the systematic development of gene-edited ruminant production.This review covers the development of gene-edited ruminants,the particulars of site-specific engineered nucleases and the state of the art and new insights into practical applications and social acceptance of genome editing technology in ruminants.It is concluded that the production of gene-edited ruminants is feasible and through improvements in genome editing technology it is possible to help feed the world.