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目的:通过研究在壳聚糖(CS)/β-磷酸三钙(β-TCP)复合体修复兔下颌骨缺损的过程中,探讨重组人骨形态发生蛋白-2(rhBMP-2)及兔面动脉血管束对成骨作用的影响,探讨CS/β-TCP/rhBMP-2+复合因子血管束修复骨缺损的可行性。方法:健康新西兰大白兔36只共36侧骨缺损(全部为左侧),随机分为3组。实验组在缺损区植入CS/β-TCP复合体,加入rhBMP-2,同时包埋兔面动脉及下颌下腺包膜组成的血管束;对照1组缺损区植入CS/β-TCP复合体,只加入rhBMP-2;对照2组缺损区植入CS/β-TCP复合体,并包埋兔面动脉及下颌下腺包膜组成的血管束,不加入rhBMP-2。于术后4周、8周、12周分批处死动物,取材后行大体观察、X线、组织切片染色观察及骨密度测定,观察各组的成骨情况。采用SPSS13.0软件包进行组间比较。结果:实验组在术后8、12周的骨密度值均显著高于对照组(P<0.05)。术后12周,实验组取材后大体观察,植入材料区皮质骨形成完好,与自体骨组织分界肉眼难辨别,X线表现较正常骨质已无明显差异,HE染色可见丰富成熟的骨小梁及板层骨,各项指标均显著优于对照组。结论:CS/β-TCP复合体修复兔下颌骨缺损过程中,加入rhBMP-2并包埋进兔面动脉血管束可明显促进成骨作用。
OBJECTIVE: To investigate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and rabbit facial arteries on the repair of mandibular defects in chickens (CS) / β-tricalcium phosphate Vascular bundles on the osteogenesis, to explore the feasibility of CS / β-TCP / rhBMP-2 + complex factor vascular bundle repair bone defects. Methods: 36 New Zealand white rabbits were divided into three groups at a total of 36 lateral defects (all on the left). The experimental group was implanted with CS / β-TCP complex in the defect area, rhBMP-2 was added, and the vascular bundle composed of rabbit articular surface and submandibular gland capsule was embedded. The control group 1 was implanted with CS / β-TCP complex , Only rhBMP-2 was added. The control group 2 was implanted with CS / β-TCP complex, and the vascular bundles consisting of rabbit arteries and submandibular gland envelopes were embedded without adding rhBMP-2. Animals were sacrificed at 4 weeks, 8 weeks and 12 weeks after operation. Gross observation, X-ray, histological staining and bone mineral density measurement were performed to observe the osteogenesis of each group. SPSS13.0 software package for comparison between groups. Results: The BMD of the experimental group at 8 and 12 weeks after operation was significantly higher than that of the control group (P <0.05). After 12 weeks of operation, the rats in the experimental group were generally observed after implantation. The formation of cortical bone in the implanted material area was intact, and it was difficult to distinguish the naked eye from the autologous bone. The X-ray showed no significant difference compared with the normal bone. HE staining showed abundant mature bone Beam and lamellar bone, the indicators were significantly better than the control group. CONCLUSIONS: Osteogenesis can be significantly enhanced by adding rhBMP-2 and embedding rabbit arterial vascular bundle in the process of repairing mandibular defects in CS / β-TCP complex.