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本文报道了研究小麦根腐叶斑病菌(Bipolars sorokiniana)在不同条件下的孢子萌发.菌丝生长和孢子产生的结果.小麦根腐叶斑病菌的孢子可在pH2.2—12.4的磷酸缓冲液中萌发,萌发的温度范围为3—39℃,最适温度22℃.菌丝生长的温度范围4—37℃,生长的pH范围为2.7—10.3,最适PH因培养基和接种方法不同而异.产孢温度11—35℃,适温20—24℃.碱性培养基(pH9以上)和黑暗下产生的孢子细而短,延迟孢子成熟,光照则促进孢子的发育. 有机氮比无机氮利于菌丝生长和产孢,以甘氨酸.丝氨酸、丙氨酸和天门冬酰胺最有利,色氨酸最差.菌丝在氨态氮上比硝态氮上生长好,但在硝态氮上产孢良好,在氨态氮上则不产孢。
This paper reports the results of spore germination, mycelial growth and spore production of wheat Biporan sorokiniana under different conditions.The spores of wheat leaf rot fungi could be cultured in phosphate buffer In the germination and germination temperature range of 3-39 ℃, the optimum temperature of 22 ℃ .The mycelial growth temperature range 4-37 ℃, the growth of the pH range of 2.7-10.3, the optimum pH due to different media and inoculation method Sporulation temperature 11-35 ℃, suitable temperature 20-24 ° C. In alkaline medium (above pH9) and spores produced in the dark thin and short, delayed spore maturation, light is to promote the development of spores. Organic nitrogen than inorganic Nitrogen favored mycelium growth and sporulation, with the most favorable for glycine, serine, alanine and asparagine, and the lowest for tryptophan. The hyphae grew better on the ammonia nitrogen than on the nitrate nitrogen, but the nitrate nitrogen On sporulation good ammonia nitrogen is not sporulation.