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以油菜为材料，在摸索出合适的种子总ＲＮＡ提取技术基础上，研究其发育过程中总ＲＮＡ量的变化，然后结合定量斑点杂交技术，研究种子发育过程中线粒体ｎａｄ６基因转录水平的变化规律。结果表明，采用的总ＲＮＡ提取方法具有操作简单、纯度高、降解少、电泳图谱理想等优点。角果宽度在２．８０ｍｍ以下的种子总ＲＮＡ量随发育而平稳地增加，角果宽度在２．８０ｍｍ以上的种子总ＲＮＡ量随发育而迅速降低。除３．８５ｍｍ角果外，发育过程中的种子总ＲＮＡ量均高于叶片的。线粒体ｎａｄ６基因的ＲＮＡ定量斑点杂交结果显示叶片ｎａｄ６基因的转录量很低，而发育中各个阶段的种子ｎａｄ６基因转录量很高，并有随发育加强的趋势。
Using rapeseed as a material, we explored the change of total RNA in the developing process by exploring the suitable technology of total RNA extraction. Then we combined with quantitative dot blot hybridization to study the variation of mitochondrial nad6 gene transcription during seed development. The results showed that the method of total RNA extraction has the advantages of simple operation, high purity, less degradation, ideal electrophoresis. The amount of total RNA in seed with a width of 2.80 mm or less increased steadily with the development, and the amount of total RNA in seeds with a width of 2.80 mm or more was rapidly decreased with development. Except for 3.85mm pod, the amount of total RNA in the seeds during development was higher than that of the leaves. Quantitative dot blot analysis of mitochondrial nad6 gene showed that the nad6 gene transcripts in leaves were very low, while the transcriptional amount of nad6 gene in all stages of development was high, with the trend of development increasing.