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采用邻苯二甲醛/9-氯甲酸芴甲酯(OPA/FMOC)柱前衍生反相高效液相色谱法(RP-HPLC),对江苏南通海区栽培的12个条斑紫菜(Porphyra yezoensis)样品游离氨基酸组成进行分析。结果显示:供试样品游离氨基酸总量在19.82～38.91mg.g-1之间,共检出16种游离氨基酸,其中Ala、Arg、Glu和Asp在所有测试样品中含量显著,占游离氨基酸总量的80%以上,Glu与Ala质量分数与游离氨基酸总量呈极显著正相关性(P<0.01);样品游离氨基酸中必需氨基酸质量分数在9.48～13.06 mg.g-1之间,占总量的33.44～52.75%;呈味氨基酸以Ala、Asp、Glu和Gly等鲜甜味氨基酸为主,质量分数在10.23～25.86 mg.g-1之间,占游离氨基酸总量的47.53～66.88%。
A total of 12 Porphyra yezoensis cultivars cultivated in Nantong sea area of Jiangsu Province were analyzed by RP-HPLC with OPA / FMOC pre-column derivatization Free amino acid composition for analysis. The results showed that there were 16 kinds of free amino acids were detected in 19.82 ~ 38.91mg.g-1, among which, Ala, Arg, Glu and Asp were significant in all tested samples, accounting for the total amount of free amino acids (P <0.01). The content of free amino acids in sample was between 9.48 ~ 13.06 mg.g-1, accounting for more than 80% The main amino acids were Ala, Asp, Glu and Gly and other sweet amino acids, with the mass fraction of 10.23 ~ 25.86 mg.g-1, accounting for 47.53 ~ 66.88% of the total free amino acids. .