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目的探讨慢病毒介导的AQP8短发卡RNA(short hairpin RNA,shRNA)对SiHa人子宫颈癌细胞迁移的影响。方法 2011年6月构建慢病毒介导的AQP8 shRNA稳定转染SiHa细胞系,新疆医科大学基础医学院病理教研室通过Transwell侵袭和迁移实验、损伤愈合实验及增殖和贴附能力实验检测AQP8基因沉默对SiHa细胞迁移的抑制作用。结果 (1)成功构建慢病毒介导的AQP8 shRNA稳定转染SiHa细胞系;(2)AQP8shRNA-SiHa侵袭迁移率明显低于对照组(F=196.451,P<0.05);AQP8shRNA-SiHa愈合速度明显低于对照组(F=37.725,P<0.05);AQP8shRNA-SiHa组增殖及基质贴附能力与对照组相比差异均无统计学意义(P>0.05)。结论抑制AQP8基因表达可以抑制SiHa细胞的迁移和潜在的侵袭过程。
Objective To investigate the effect of lentivirus-mediated AQP8 short hairpin RNA (shRNA) on the migration of SiHa human cervical carcinoma cells. Methods AQP8 shRNA was transfected into SiHa cell line stably transfected with lentivirus-mediated AQP8 shRNA in June 2011. Transwell invasion and migration assay, wound healing assay and proliferation and adhesion assay were used to detect AQP8 gene silencing Inhibition of SiHa cell migration. Results (1) The lentivirus-mediated AQP8 shRNA was successfully constructed and transfected into SiHa cells. (2) The invasion and migration of AQP8shRNA-SiHa was significantly lower than that of the control group (F = 196.451, P <0.05) (F = 37.725, P <0.05). There was no significant difference in proliferation and matrix attachment between AQP8shRNA-SiHa group and control group (P> 0.05). Conclusion Inhibition of AQP8 gene expression can inhibit SiHa cell migration and potential invasion.