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目的研究花锚提取物中3种酮苷对肝脏保护作用。方法用CC l4灌胃小鼠,建立急性肝损伤动物模型,观察其肝脏组织形态,测定肝脏系数,血清中谷丙转氨酶、谷草转氨酶活性和肝糖元含量,研究3种酮苷对小鼠肝损伤的保护作用。结果模型组中小鼠的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性、肝脏系数、肝糖元A值分别为(1045.61±47.82),(1323.52±230.22)U/L,(0.062±0.0041),(0.062±0.013);与模型组比较,花锚中3种酮苷高剂量组(10 mg/m l)对小鼠实验性肝损伤均有明显的修复作用,能降低CC l4所致肝损伤小鼠的肝脏系数、血清ALT、AST活性,并提高肝糖元含量(P<0.01);化合物A、B、C中剂量组(5 mg/m l)的肝糖元A值分别为(0.072±0.011),(0.077±0.015),(0.078±0.012),与模型组相比均明显升高(P<0.05),其余各项指标与模型组比较,差异均有统计学意义(P<0.01);与模型组比较,化合物B低剂量组(1 mg/m l)可有效降低小鼠的肝脏系数及血清AST活性(P<0.05),化合物C低剂量组可明显降低小鼠血清AST活性(P<0.01),对ALT活性的升高也有较好的抑制作用(P<0.05),而其他给药组与模型组比较,差异均无统计学意义。结论花锚中3种酮同苷均有较好的保肝作用,为花锚保护肝脏的有效成分、且具有一定的量效关系。
Objective To study the liver protective effects of three kinds of ketosides in Huagan extract. Methods The mice were intragastrically administered with CC l4 to establish an animal model of acute hepatic injury. The liver tissue morphology, hepatic index, serum glutamic-pyruvic transaminase, aspartate aminotransferase and hepatic glycogen were determined. The effects of three ketosides on liver injury in mice were studied. Protection. Results Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity, hepatic coefficient, and hepatic glycogen A value in mice in the model group were (1045.61±47.82), (1323.52±230.22) U/L, (0.062±0.0041, respectively). ), (0.062±0.013); Compared with the model group, the three high-dose ketoside groups (10 mg/ml) in the flower anchor have obvious repair effect on experimental liver injury in mice and can reduce the liver caused by CC l4. The hepatic index, serum ALT, AST activity and hepatic glycogen content in mice were increased (P<0.01). The hepatic glycogen A values in the dose group (5 mg/ml) in compound A, B, and C were (0.072). ±0.011), (0.077±0.015), (0.078±0.012), significantly increased compared with the model group (P<0.05), and the other indicators were statistically significant compared with the model group (P<0.01). Compared with the model group, Compound B low-dose group (1 mg/ml) can effectively reduce liver coefficient and serum AST activity in mice (P<0.05). Compound C low-dose group can significantly reduce serum AST activity in mice. P<0.01), also had better inhibitory effect on the increase of ALT activity (P<0.05), while there was no statistically significant difference between the other groups and the model group. Conclusion The three kinds of ketone glycosides in flower anchors have better hepatoprotective effect, and they are the active ingredients of flower anchors to protect the liver and have a certain dose-effect relationship.