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目的采用MTS法评价五种燃煤PM2.5对血管内皮细胞EA.hy926的细胞毒性。方法以织金散煤、京西散煤、银川散煤、大同散煤及东胜散煤五种煤为样煤,测定五种煤的挥发分产率,在实验室采用固定源稀释通道采集燃煤PM2.5,分别对人脐静脉内皮细胞EA.hy926进行染毒,采用MTS法检测五种燃煤PM2.5对EA.hy926细胞增殖的影响,并对五种燃煤的PM2.5排放因子进行探索性分析。结果五种燃煤PM2.5均可抑制血管内皮细胞增殖,且相同剂量条件下,五种燃煤PM2.5对EA.hy926细胞的细胞毒性大小具有银川=东胜>大同>织金>京西的趋势,但差异无统计学意义,且五种燃煤PM2.5排放因子具有基本一致的趋势。结论煤种虽然来自不同地域,挥发分也不相同,但其对血管内皮细胞的毒性无差异性,提示改善燃煤释放PM2.5对健康效应影响,必须采取改变能源消耗结构和降低燃煤用量等相关措施
Objective To evaluate the cytotoxicity of five kinds of coal-fired PM2.5 on human vascular endothelial cell line EA.hy926 by MTS method. Methods Volatile matter yield of five kinds of coal was measured with five kinds of coal, namely Zhijin bulk coal, Beijing Xiansi coal, Yinchuan bulk coal, Datong bulk coal and Dongsheng scattered coal. The fixed source dilution channel was used in the laboratory to collect Coal-fired PM2.5, respectively, human umbilical vein endothelial cells EA.hy926 were exposed to MTS method to detect five kinds of coal-fired PM2.5 EA.hy926 cells proliferation, and five kinds of coal-fired PM2.5 Emission factors for exploratory analysis. Results Five kinds of coal-fired PM2.5 could inhibit the proliferation of vascular endothelial cells, and under the same dosage, the cytotoxicity of five kinds of coal-fired PM2.5 on EA.hy926 cells had Yinchuan = Dongsheng> Datong> Zhijin> Jing West trend, but the difference was not statistically significant, and the five kinds of coal-fired PM2.5 emission factors have basically the same trend. Conclusion Although the coal species come from different regions and have different volatile components, their toxicity to vascular endothelial cells is no different, suggesting that to improve the effect of PM2.5 release on the health effects of coal combustion, the energy consumption structure must be changed and the coal consumption reduced And other related measures