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1994~1996年间,共选用6种胞质类型的12个不育系及其相应的保持系,6个同核异质材料和携带不同抗性基因的品种和杂交组合,于水稻孕穗期接种分属于7个致病型的白叶枯病菌35个菌株。结果表明,不育胞质与正常胞质的抗病性程度大体相近,但在少数不育胞质与部分白叶枯病菌菌株的互作中,表现出明显的胞质效应,即不育系较之保持系更感病,主要表现在野败型的珍汕97和V20、冈型的G汕、D型的D汕等几种不育胞质与Ah28、Gx50等部分菌株的特异性互作体系中;6个同核异质水稻抗病性也具有明显差异,Ah28等部分菌株对D汕A1和G汕A等胞质具有较强的亲和性;杂交稻的抗病性主要受核基因控制,但胞质亦有一定影响。因此,在杂交稻抗白叶枯病育种中,胞质效应是一个不可忽视的因素,在改良核基因的同时,要注重胞质的多样化。
From 1994 to 1996, a total of 12 CMS lines with their corresponding maintainer lines, 6 isonuclear heterogeneous materials and varieties and crosses with different resistance genes were selected and inoculated at the booting stage of rice Belonging to seven pathogenic bacterial blight bacteria 35 strains. The results showed that the resistance of sterile cytoplasm to normal cytoplasm was similar, but the cytoplasmic effect was observed in the interaction between a few sterile cytoplasm and some bacterial blight strains, Compared with the maintainer lines, the two lines mainly expressed in the wild-type Zhenshan 97 and V20, Gang type G Shantou, D-type D Shantou and several other sterile cytoplasm and Ah28, Gx50 and some other strains of the specific interaction The resistance of six iso-nuclear isolates also showed significant differences. Some strains such as Ah28 had strong affinity to cytoplasm such as D-Shan A1 and G-Shany A, and the resistance of hybrid rice was mainly affected by nuclear Gene control, but also a certain impact on the cytoplasm. Therefore, in hybrid rice bacterial blight resistance breeding, cytoplasmic effect is a non-negligible factor in the improvement of nuclear genes, we must pay attention to the diversity of the cytoplasm.