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为了探究二十二碳六烯酸(docosahexaenoic acid,DHA)抗乳腺癌的机制,采用流式细胞术分析细胞死亡率,H2DCFDA探针流式细胞术测定细胞内活性氧(reactive oxygen species,ROS)水平,Western blotting方法检测细胞内剪切型Caspase-3的变化。结果发现在无葡萄糖条件下,DHA以浓度依赖性方式促进乳腺癌细胞MDA-MB-231死亡。在这个过程中,DHA促使细胞内ROS水平明显升高,然而,当DHA和抗氧化剂N-乙酰-半胱氨酸(N-acetyl-L-cystein,NAC)共处理细胞时,细胞死亡率和细胞内ROS水平均明显降低。Western blotting检测结果表明DHA可以引起Caspase-3的切割,同时,这种变化可以被NAC抑制。结果表明在无葡萄糖条件下,DHA可以通过增加细胞内ROS的水平,激活Caspase凋亡通路,进而促进乳腺癌细胞MDA-MB-231死亡。
In order to explore the mechanism of docosahexaenoic acid (DHA) against breast cancer, the cell death rate was analyzed by flow cytometry. The activity of reactive oxygen species (ROS) was measured by H2DCFDA probe flow cytometry. The changes of intracellular shear-type Caspase-3 were detected by Western blotting. DHA was found to promote MDA-MB-231 breast cancer cell death in a concentration-dependent manner in the absence of glucose. In the process, DHA promoted the intracellular ROS level to increase significantly. However, when DHA co-treated with antioxidant N-acetyl-L-cystein (NAC), the cell death rate and Intracellular ROS levels were significantly reduced. Western blotting results showed that DHA can cause Caspase-3 cleavage, meanwhile, this change can be inhibited by NAC. The results showed that in the absence of glucose, DHA can activate the Caspase apoptosis pathway by increasing the intracellular ROS level, and then promote the death of breast cancer cells MDA-MB-231.