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目的对陕西省健康儿童肠道病毒(Enterovirus,EV)感染情况进行分析,为EV所引起疾病的预防与控制提供参考。方法按世界卫生组织《脊髓灰质炎实验室操作手册》规程进行病毒分离,用逆转录-聚合酶链反应扩增分离到的病毒VP1编码区基因片段,并进行核苷酸序列测定和分析,以鉴定EV的型别。结果对健康儿童159份粪便标本进行病毒分离,其中34份病毒分离阳性,阳性率21.38%。在阳性标本中,埃柯病毒(Enteric Cytopathogenic Human Orphan Virus,ECHO;引起细胞病变的人肠道孤儿病毒)7型(ECHO7)11份,占32.35%;柯萨奇病毒(Coxsackie Virus,CV)B组5型(CVB5)5份,占14.71%;ECHO14、CVB3各3份,各占8.82%;CVA92份,占5.88%;ECHO30、ECHO13各1份,各占2.94%;另外8株病毒未能确定型别。结论陕西省健康儿童中ECHO7感染率最高,且不同地区所分离的EV型别不同,增加了对EV所引起的疾病进行预防与控制的难度。
Objective To analyze the status of Enterovirus (EV) infection in healthy children in Shaanxi Province and provide references for the prevention and control of diseases caused by EV. Methods Virus isolation was performed according to the “Polio Laboratory Operation Manual” of the World Health Organization (WHO). The isolated VP1 coding region of the virus was amplified by reverse transcription - polymerase chain reaction (PCR) and the nucleotide sequence was determined. Identify the type of EV. Results 159 stool samples from healthy children were isolated by virus, of which 34 were positive for virus isolation and the positive rate was 21.38%. In positive samples, 11 cases of Enteric Cytopathogenic Human Orphan Virus (ECHO; cytopathogenic human enterovirus) type 7 (ECHO7) accounted for 32.35%; Coxsackie Virus (CV) B 5 for CVB5, accounting for 14.71%; 3 for each of ECHO14 and CVB3, accounting for 8.82%; 92 for CVA, accounting for 5.88%; 1 for each of ECHO30 and ECHO13, accounting for 2.94%; 8 for other viruses failed Determine the type. Conclusions The highest infection rate of ECHO7 among healthy children in Shaanxi Province is that the EV type isolated in different areas is different, which makes it more difficult to prevent and control the diseases caused by EV.