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目的:建立喉咽清口服液中齐墩果酸-28-O-β-D-吡喃葡萄糖苷的含量测定方法。方法:色谱柱为Hibar C_(18)(250 mm×4.6 mm,5μm),乙腈-0.4%磷酸(34:66)为流动相;流速为1.0 ml·min~(-1);检测波长为203 nm。结果:齐墩果酸-28-O-β-D-吡喃葡萄糖苷在0.146～4.848μg(r=1.000 0)范围内线性关系良好,平均回收率为98.86%,RSD为1.62%(n=6)。结论:所建立的方法简便,准确,为该制剂质量标准的建立提供一定的参考。
Objective: To establish a method for the determination of oleanolic acid-28-O-β-D-glucopyranoside in Huanghuang Oral Liquid. METHODS: The mobile phase consisted of Hibar C 18 (250 mm × 4.6 mm, 5 μm) and acetonitrile-0.4% phosphoric acid (34:66) with a flow rate of 1.0 ml · min -1. The detection wavelength was 203 nm. Results: The linearity of oleanolic acid-28-O-β-D-glucopyranoside in the range of 0.146-4.848μg (r = 1.000 0) was good with the average recovery of 98.86% and RSD of 1.62% (n = 6). Conclusion: The established method is simple and accurate, which can provide reference for the establishment of the quality standard of the preparation.