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目的：探讨哮喘患儿自然杀伤细胞及γ干扰素水平的变化及意义。方法：应用四甲基偶氮唑盐（ＭＴＴ）比色法、流式细胞术（ＦＣＭ）及生物素亲合素双抗体夹心酶联免疫吸附分析术（ＡＢＣＥＬＩＳＡ）检测哮喘患儿外周血自然杀伤细胞（ＮＫＣ）活性、ＣＤ５６＋细胞数及单个核细胞（ＰＢＭＮＣ）诱生γ干扰素（ＩＦＮγ）水平。结果：哮喘发作期及缓解期ＮＫＣ活性与正常对照比较均无显著差异（Ｐ＞０．０５）；发作期ＣＤ５６＋细胞数明显低于正常对照（Ｐ＜００１），后者与缓解期无显著性差异（Ｐ＞００５）；ＰＢＭＮＣ诱生ＩＦＮγ水平发作期及缓解期均显著低于正常对照（Ｐ＜０．０１）。结论：ＩＦＮγ产生减少可能与哮喘的发生和易诱发病毒反复感染有一定关系；而ＮＫ细胞活性在发病过程中变化不大。
Objective: To investigate the changes and significance of natural killer cells and interferon-γ in children with asthma. Methods: The peripheral blood of asthmatic children was detected by MTT colorimetric assay, flow cytometry (FCM) and biotin-avidin double antibody sandwich enzyme-linked immunosorbent assay (ABCELISA) NKC activity, the number of CD56 + cells and the level of IFN-γ induced by PBMNC. Results: There was no significant difference in NKC activity between the onset and remission phases of asthma and normal controls (P> 0.05). The number of CD56 + cells in the attack phase was significantly lower than that in the normal control group (P <001) (P> 0.05); PBMNC-induced IFN-γ levels were significantly lower than the normal control (P <0.01) at the onset and remission stage. Conclusion: The decrease of IFNγ production may be related to the occurrence of asthma and the susceptibility to recurrent viral infection. However, the activity of NK cells changes little during the course of the disease.