论文部分内容阅读
目的筛选铜绿假单胞菌野生型菌株PAO1对左氧氟沙星耐药前后的差异蛋白质,探讨这些蛋白质与铜绿假单胞菌对左氧氟沙星耐药的关系。方法采用亚抑菌浓度梯度递增法体外诱导PAO1对左氧氟沙星耐药,并设置同步对照组,应用表面增强激光解析电离飞行时间质谱(SELDI)技术和CM10蛋白质芯片检测PAO1对左氧氟沙星耐药株和同步对照菌株的菌体蛋白,采用Biomarker Wizard软件进行分析。结果左氧氟沙星耐药株与同步对照菌株间有8个差异蛋白(P<0.05),5个差异蛋白高表达,分子量分别为1 004、1 007、1 918、5 175Da和5 454Da;有3个蛋白质低表达,分子量分别为1 012、1 629、2 004Da。结论用蛋白质芯片和SELDI技术对PAO1的左氧氟沙星耐药菌株与同步对照菌株进行蛋白质组学研究,可以筛选出与PAO1对左氧氟沙星耐药可能相关的差异蛋白质。
Objective To screen the differential proteins before and after resistance to levofloxacin of Pseudomonas aeruginosa wild-type strain PAO1, and to explore the relationship between these proteins and resistance to levofloxacin of Pseudomonas aeruginosa. Methods PAO1 was induced to levofloxacin in vitro by gradient-increasing method of sub-bacteriostatic concentration. Synchronized control group was set up. Surface plasmon resonance ionization time-of-flight mass spectrometry (SELDI) and CM10 protein chip were used to detect the effect of PAO1 on levofloxacin- Strain bacterial protein, using Biomarker Wizard software for analysis. Results There were 8 differential proteins between levofloxacin-resistant and synchronous control strains (P <0.05), and 5 differential proteins were highly expressed with molecular weights of 1 004, 1 007, 1 918, 5 175 and 5 454 Da respectively. There were 3 proteins Low expression, molecular weight respectively 1 012,1 629,2 004Da. Conclusion The proteomics study of the levofloxacin-resistant strains of PAO1 and the synchronous control strains using protein chip and SELDI technique can screen out the differential proteins that may be related to the drug resistance of levofloxacin to PAO1.