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目的:基因是受体酪氨酸激酶(RTK)基因家族中最大的亚族。文中通过CpG岛高甲基化是导致EphA7基因在结直肠癌中表达下调的重要机制。检测EphA7基因mRNA在胃癌组织中的表达水平,探讨其对胃癌发生、发展及预后的意义。方法:利用荧光标记探针进行实时定量RT-PCR,测定62例胃癌患者的癌组织及癌旁非癌组织标本中EphA7 mRNA表达水平,结合临床病理学指标进行统计学处理,分析EphA7基因在胃癌组织和正常胃黏膜中表达水平与临床病理资料之间的关系。结果:EphA7基因表达水平在不同胃癌患者的癌组织标本和癌旁非癌胃黏膜中呈现差异性表达。根据EphA7在癌组织和癌旁非癌胃黏膜中的表达水平,可分为3种类型:正常黏膜/癌组织mRNA表达比值(N/T)(0.5为表达上调(22/62),(2为表达下调(27/62),介于0.5和2之间为表达无差异(13/62)。EphA7 mRNA表达与患者年龄有关(P=0.030),亦与肿瘤分期有关(P=0.031)。EphA7表达上调与肿瘤转移有一定的相关性(P=0.063)。结论:EphA7基因表达水平在不同的胃癌患者中有很大差异。EphA7基因表达上调可能在胃癌的发生和疾病进展中起一定作用。
Purpose: Genes are the largest subfamily of receptor tyrosine kinase (RTK) gene families. The hypermethylation of CpG islands is an important mechanism for the down-regulation of EphA7 gene expression in colorectal cancer. To detect the expression of EphA7 mRNA in gastric cancer tissues and to explore its significance for the occurrence, development and prognosis of gastric cancer. Methods: Real-time quantitative RT-PCR was performed using fluorescently labeled probes to determine the expression level of EphA7 mRNA in cancer tissues and adjacent noncancerous tissues of 62 patients with gastric cancer. The clinical pathological parameters were used for statistical analysis to analyze the EphA7 gene in gastric cancer. The relationship between the expression level and clinical pathological data in tissues and normal gastric mucosa. RESULTS: The expression level of EphA7 gene was differentially expressed in the specimens of cancer tissues and non-cancerous gastric mucosa of different gastric cancer patients. According to the expression level of EphA7 in cancer tissue and adjacent non-cancerous gastric mucosa, it can be divided into three types: normal mucosa/cancer tissue mRNA expression ratio (N/T) (0.5 is up-regulated (22/62), (2 Down-regulation (27/62), with no difference between 0.5 and 2 (13/62), EphA7 mRNA expression was correlated with patient age (P=0.030) and also with tumor stage (P=0.031). The upregulation of EphA7 expression correlated with tumor metastasis (P=0.063).Conclusion: The expression level of EphA7 gene is different in different gastric cancer patients.Upregulation of EphA7 gene expression may play a role in the occurrence of gastric cancer and disease progression. .