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目的探讨端粒酶在原发性胆囊癌中的活性及临床意义。方法采用端粒重复序列扩增法(TRAP)检测了胆囊癌细胞系,46例原发性胆囊癌组织,22例癌旁组织,19例胆囊良性病变。结果胆囊癌细胞系呈端粒酶阳性,癌组织的端粒酶阳性率为84.8%,显著高于癌旁组织和胆囊良性病变(P>0.05)。端粒酶活性与胆囊癌病理类型和分化程度无相关性(P>0.05)。与浸润深度和淋巴结转有显著相关性(P<0.05)。结论端粒酶激活与原发性胆囊癌的发生、发展有密切关系,并有可能成为原发性胆囊癌的临床肿瘤标记物。
Objective To investigate the activity and clinical significance of telomerase in primary gallbladder carcinoma. Methods TLR was used to detect gallbladder carcinoma cell lines, 46 cases of primary gallbladder carcinoma, 22 cases of paracancerous tissues, and 19 cases of benign gallbladder lesions. RESULTS: Gallbladder cancer cell lines were positive for telomerase and the positive rate of telomerase in cancer tissues was 84.8%, which was significantly higher than that in adjacent tissues and gallbladder benign lesions (P>0.05). There was no correlation between telomerase activity and pathological types and differentiation of gallbladder carcinoma (P>0.05). There was a significant correlation between infiltration depth and lymph node metastasis (P<0.05). Conclusion Telomerase activation is closely related to the occurrence and development of primary gallbladder carcinoma, and may be a clinical tumor marker for primary gallbladder cancer.