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研究了同时测定血清、尿样中的咖啡因和茶碱的薄层色谱定量法。在ＧＦ２５４硅胶板上，用乙酸乙酯－氨水（Ｖ乙酸乙酯∶Ｖ氨水＝９６∶４）的二元展开体系能将这两种生物碱有效地分离。其Ｒｆ值为咖啡因０．４６，茶碱０．１６。分离后的试样用薄层色谱扫描仪进行扫描定量，测定波长２７２ｎｍ，线性范围咖啡因０．００９７～１．９４μｇ，茶碱０．００９０～１．８０μｇ。血清及尿样的加标回收率在９５．８％～１０６．５％之间，相对标准偏差２．９５％～３．５０％。本法较为简便、快速、准确
The simultaneous determination of caffeine and theophylline in serum and urine samples by TLC was studied. On GF254 silica gel plates, the two alkaloids were efficiently separated by a binary unwrapped system with ethyl acetate-ammonia (V ethylacetate: V ammonia = 96: 4). The Rf value for caffeine 0.46, theophylline 0.16. The separated sample was scanned and quantified with a thin-layer chromatography scanner. The measurement wavelength was 272 nm. The linear range was 0.0097 to 1.94 μg of caffeine and 0.0090 to 1.80 μg of theophylline. The spiked recoveries of serum and urine samples ranged from 95.8% to 106.5% with relative standard deviations of 2.95% -3.50%. This law is relatively simple, fast and accurate