【摘 要】
：
cAMP 反应元件结合蛋白(cAMP response element-binding proteins,CREB)是一个哺乳动物转录因子家族,通过cAMP 反应元件(cAMP response element,CRE)介导cAMP和钙离子依赖性
【机 构】
：
华东师范大学生命科学院生物化学及分子生物学实验室,上海新智生物技术有限公司,复旦大学生命科学院遗传所遗传工程国家重点实验室
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cAMP 反应元件结合蛋白(cAMP response element-binding proteins,CREB)是一个哺乳动物转录因子家族,通过cAMP 反应元件(cAMP response element,CRE)介导cAMP和钙离子依赖性基因表达.CREB4是CREB转录家族的新成员.人肿瘤MTC panel结果显示,CREB4在人肺癌LX-1、结肠腺癌CX-1、前列腺癌PC-3、结肠癌G1-112和胰腺癌G1-103中有表达.构建表达CREB4-LexA和CREB215～395aa-LexA的pLexA融合质粒分别转化含p8opLacZ报告质粒的酵母EGY48菌株,诱导表达后发现CREB4蛋白为转录激活因子,N端决定其转录激活活性.亚细胞定位结果显示,全长CREB4蛋白定位于细胞质,而缺失C端假定转膜结构域的CREB41～275aa蛋白突变体则转移至细胞核内.表达谱结果显示CREB4蛋白可能在人多种肿瘤组织的基因表达调控中起作用,其C端假定的转膜结构域与其转录激活功能密切相关.
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