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采用牙周膜成纤维细胞体外培养，观察多聚腺嘌呤二磷酸核糖合成酶抑制剂及诱导剂对体外培养的牙周膜成纤维细胞α１（１）和α１（Ⅲ）胶原基因ｍＲＮＡ水平的影响，α１（Ⅰ）和α１（Ⅲ）胶原基因ｃＤＮＡ探针分别来自Ｈｆ６７７和Ｈｆ９３４。结果发现，多聚腺嘌呤二磷酸核糖合成酶抑制剂（１０ｍｍｏｌ／Ｌ尼克酰胺，５ｍｍｏｌ／Ｌ３－氨基苯胺和２０ｍｍｏｌ／Ｌ乳酸钠）都能明显增加α１（Ⅰ）和α１（Ⅲ）胶原基因ｍＲＮＡ水平，而多聚腺嘌呤二磷酸核糖合成酶诱导剂（１ｍｍｏｌ／Ｌ 辅酶Ⅰ）则明显降低α１（Ⅰ）和α１（Ⅲ）胶原基因ｍＲＮＡ水平。提示可以通过抑制多聚腺嘌呤二磷酸核糖的合成促进牙周膜成纤维细胞胶原的合成，这为牙周组织再生的研究提供了新的方向。
The effects of poly-ADP synthase inhibitors and inducers on the mRNA expression of α1 (1) and α1 (Ⅲ) collagen in periodontal ligament fibroblasts cultured in vitro were studied by using periodontal ligament fibroblasts cultured in vitro , Α1 (Ⅰ) and α1 (Ⅲ) collagen gene cDNA probes were from Hf677 and Hf934, respectively. The results showed that poly (ADP-ribose) synthase inhibitors (10 mmol / L nicotinamide, 5 mmol / L 3-aminoaniline and 20 mmol / L sodium lactate) significantly increased the mRNA levels of α1 (Ⅰ) and α1 (1mmol / L coenzyme Ⅰ) significantly decreased the mRNA levels of α1 (Ⅰ) and α1 (Ⅲ) collagen. It is suggested that the synthesis of collagen in periodontal ligament fibroblasts can be promoted by inhibiting the synthesis of poly-ADP ribose, which provides a new direction for the study of periodontal tissue regeneration.