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目的:研究2种类黄酮化合物白藜芦醇和芦丁对人单核及多核白细胞分泌白介素8(IL-8)和白介素6(IL-6)的抑制作用,探讨其对于治疗脓毒血症患者是否具有临床意义。方法:采10例健康人静脉血,分离白细胞,加入20μmol.L-1白藜芦醇或50μmol.L-1芦丁,在37℃和5%CO2条件孵育箱培养4 h,加入20 mg.L-1脂多糖(LPS)或20μmol.L-1肽聚糖(PGN),培育过夜后测定培养基中IL-8和IL-6浓度,分别与阳性对照(只在细胞中加入LPS或PGN)和DMSO阴性对照比较。结果:芦丁和白藜芦醇均显著降低LPS刺激后单核细胞的IL-8分泌量(n=10,P<0.01),以及PGN刺激后多核细胞的IL-6分泌量(n=10,P<0.05)。结论:白藜芦醇和芦丁对人单核细胞及多核细胞分泌IL-8和IL-6的有抑制作用,这种抑制作用可能具有细胞特异性。
Objective: To study the inhibitory effects of two kinds of flavonoids, resveratrol and rutin, on the secretion of interleukin-8 (IL-8) and interleukin-6 (IL-6) by human mononuclear and polynuclear leukocytes, and to investigate whether they are effective in the treatment of sepsis patients. Has clinical significance. METHODS: Ten healthy human venous blood samples were collected and leukocytes were isolated. After adding 20 μmol·L-1 resveratrol or 50 μmol·L-1 rutin, the cells were incubated at 37°C and 5% CO2 for 4 h and 20 mg was added. L-1 lipopolysaccharide (LPS) or 20 μmol.L-1 peptidoglycan (PGN) was assayed overnight after incubation for IL-8 and IL-6 concentrations in culture media, with positive controls (LPS or PGN only in cells) ) Comparison with DMSO negative controls. RESULTS: Both rutin and resveratrol significantly reduced the secretion of IL-8 from LPS-stimulated monocytes (n=10, P<0.01) and the secretion of IL-6 from PGN-stimulated multinuclear cells (n=10). P<0.05). CONCLUSION: Resveratrol and rutin have inhibitory effects on the secretion of IL-8 and IL-6 from human monocytes and multinuclear cells. This inhibition may be cell-specific.