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目的:本研究拟同时检测survivin与p27两种蛋白质在非霍奇金淋巴瘤(NHL)组织中的表达水平,探讨其相互关系以及与部分临床指标的关系及其意义。方法:选择51例NHL患者的组织标本,30例反应性淋巴结炎标本作为对照组,采用免疫组化方法,观察在NHL组织中survivin及p27的表达有无异常;并研究二者之间的关系。结果:NHL组织中同时存在survivin和p27基因表达异常,在NHL组织中survivin蛋白质与p27蛋白质的表达呈负相关(P<0.01)。结论:在NHL组织中同时有survivin和p27基因表达异常,提示应用分子生物学方法,有可能从不同角度靶向survivin和(或)p27基因进行抗肿瘤治疗,更好地发挥抗肿瘤效应;对进一步研究将正常p27基因导入NHL的治疗或针对survivin基因的靶向治疗有较大的启发和帮助。
OBJECTIVE: In this study, the expression levels of survivin and p27 proteins in non-Hodgkin’s lymphoma (NHL) tissues were determined simultaneously to explore their relationship and their relationship with some clinical indicators. Methods: Tissues from 51 patients with NHL and 30 patients with reactive lymphadenitis were selected as control group. Immunohistochemistry was used to observe the expression of survivin and p27 in NHL. The relationship between the two . Results: There were both survivin and p27 gene expression in NHL tissues. There was a negative correlation between the expression of survivin protein and p27 protein in NHL tissues (P <0.01). CONCLUSIONS: The abnormal expression of both survivin and p27 in NHL suggests that it is possible to target anti-tumor therapy of survivin and / or p27 gene from different angles and to exert anti-tumor effect better by using molecular biology method. Further study of the normal p27 gene into the treatment of NHL or targeted therapy for survivin gene have greater inspiration and help.