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目的：制备珊瑚（ｃｏｒａｌ）与重组人骨形成蛋白－２（ｒｈＢＭＰ－２）复合人工骨并测试其骨诱导活性。方法：将ｒｈＢＭＰ－２与珊瑚复合后植入鼠肌袋内。６６只小鼠随机分为３组：第１组为复合骨（１∶２０ｗ／ｗ）；第２组为复合骨（１∶４０ｗ／ｗ）；第３组为单独珊瑚。术后１、３、６周处死动物，光镜下观察，并用图象分析法作成骨定量测定，结果：术后１周，可见复合材料表面及孔洞内有软骨形成。３周出现编织骨。６周形成含骨髓的板层骨。珊瑚被部分吸收。诱导成骨的量有时间依赖性和ｒｈＢＭＰ－２剂量依赖性。结论：ｒｈＢＭＰ－２／ｃｏｒａｌ复合人工骨具有良好的诱导成骨能力；珊瑚可能是目前能使用的ｒｈＢＭＰ－２最合适的缓释载体。
OBJECTIVE: To prepare coral and recombinant human bone morphogenetic protein-2 (rhBMP-2) composite artificial bone and test its osteoinductive activity. Methods: The rhBMP-2 complexed with coral implantation in the rat muscle bags. 66 mice were randomly divided into three groups: the first group was composite bone (1:20w / w); the second group was composite bone (1:40w / w); the third group was single coral. The animals were sacrificed at 1, 3 and 6 weeks after operation, and observed under light microscope. Quantitative analysis of the bone was performed by image analysis. Results: After 1 week, the cartilage formed on the surface of the composite and in the hole. Braided bone appeared in 3 weeks. 6 weeks to form bone marrow-containing lamellar bone. Corals are partially absorbed. The amount of induced osteogenesis was dose-dependent and rhBMP-2 dose-dependent. CONCLUSION: rhBMP-2 / coral composite artificial bone has good ability of inducing osteogenesis. Coral may be the most suitable sustained release carrier of rhBMP-2 currently available.