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松树与松材线虫互作中,NO作为重要的信号分子参与调控寄主对松材线虫入侵的应答反应,但松材线虫入侵寄主后,是松材线虫还是其代谢产物激发寄主体内NO应答信号尚不明确.本研究用松材线虫活体、线虫分泌物、虫体研磨液等分别接种黑松,研究接种早期黑松体内NO合成酶(NOS)活性和NO含量变化与后期松树症状发展的关系.结果表明:采用虫体研磨液、线虫分泌物处理后黑松体内NOS活性和NO含量均升高;各处理黑松外部均出现感病症状.说明在松树与松材线虫互作中,除线虫活体作用外,线虫体或分泌物中的物质也可激发松树体内NO应答信号的表达,诱导下游应答响应的发生,导致黑松感病.15～25℃条件下,随着温度升高,接种黑松体内NOS活性增强,NO含量升高,感病症状出现较早;随着干旱胁迫程度的增强,接种黑松体内NOS活性、NO含量大致呈逐步升高趋势,感病症状也较早出现.在一定范围内,温度升高和干旱程度加剧可诱发感病黑松体内NO相关信号途径表达增强,并加速病程发展.表明在一定条件下,高温干旱环境有利于松材线虫病的发生可能与寄主体内NO应答信号增强有关.
In the interactions between pine and pine wood nematode, NO as an important signaling molecule participates in the regulation of host response to the pine wood nematode invasion. However, whether pine wood nematode invades the host or not and the metabolites stimulate NO responses in the host In this study, black pine was inoculated with live pine wood nematode, nematode secretion and larvae, respectively, to study the relationship between the changes of NO synthase (NOS) activity and NO content and the development of later pine symptoms in early stage of inoculation. The results showed that NOS activity and NO content increased after treatment with nematode secretion and nematode secretion, and all the symptoms of the disease were observed in the outer part of the treated P. nigricans, indicating that in addition to nematodes In vivo, the nematodes or secretions can also stimulate the NO response signal in the pine and induce the response of the downstream response, leading to the susceptible to the disease.When the temperature is raised from 15 ℃ to 25 ℃, inoculation NOS activity of black pine increased, the content of NO increased, the symptom appeared earlier; with the increase of drought stress, the activity of NOS was inoculated in black pine, the content of NO increased gradually and the symptom was earlier Appeared in a certain range, increased temperature and increased degree of drought can induce the expression of NO-related signal pathway in susceptible bryophytes increased, and accelerate the course of development.It shows under certain conditions, high temperature and drought environment is conducive to the occurrence of pine wilt disease May be related to the host NO response signal enhancement.