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通过气相色谱法对小鼠血和脑组织中1,2-二氯乙烷(1,2-DCE)和氯乙酸的浓度进行检测,同时对小鼠进行脑损伤行为学观察和脑含水量的测定。结果显示小鼠血和脑组织中的1,2-DCE浓度在染毒结束后20 min内分别下降74.16%和64.15%,半减期分别为16.2 min和18.7 min。血和脑组织中氯乙酸浓度在染毒期间快速升高,在染毒结束时达到峰值,染毒结束后40 min内氯乙酸浓度相对稳定,但之后迅速下降,至1 h时,血和脑组织中氯乙酸浓度分别下降至浓度最高时的11.45%和13.72%。脑损伤行为学观察异常的小鼠表现为抱爪现象,抱爪小鼠血中氯乙酸含量明显高于不抱抓小鼠。小鼠脑含水量与氯乙酸的浓度呈显著正相关,相关系数为0.77。提示1,2-DCE的体内代谢程度是影响小鼠脑水肿形成的重要因素。
The concentrations of 1,2-dichloroethane (1,2-DCE) and chloroacetic acid in blood and brain tissues of mice were detected by gas chromatography. The brain damage behavior and brain water content Determination. The results showed that the concentrations of 1,2-DCE in blood and brain tissue of mice decreased by 74.16% and 64.15% respectively within 20 min after the end of treatment, with the half-reductions of 16.2 and 18.7 min, respectively. The concentration of chloroacetic acid in blood and brain tissue increased rapidly during exposure and peaked at the end of exposure. The concentration of chloroacetic acid was relatively stable within 40 min after the end of the exposure, but then decreased rapidly. After 1 h, blood and brain Tissue concentrations of chloroacetic acid decreased to 11.45% and 13.72%, respectively, at the highest concentrations. The mice with abnormal brain injury behavior observation showed the phenomenon of holding the claws. The content of chloroacetic acid in the blood of the clawed mice was obviously higher than that of the mice without grasping. There was a significant positive correlation between brain water content and the concentration of chloroacetic acid in mice, with a correlation coefficient of 0.77. Tip 1,2-DCE in vivo metabolism is an important factor affecting the formation of cerebral edema in mice.