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This paper reports a new method to prepare chitosan membrane which could be used as a biomedical material.Addition of a fixation agent composed of alcohol, glycerol and potassium hydroxide can accelerate the sol-gel transformation process and hence shorten the preparation period. The present method takes about 6 h to get a flexible membrane with fine appearance. The physical and biological properties of the membrane were also investigated and compared with the membrane prepared by conventional method.``