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扫描离子电导显微镜技术是纳米生物医学领域新近发展起来的一种扫描探针显微镜技术,可被用来在生理条件下、高分辨率及非接触地研究活细胞的表面形貌。扫描用玻璃微探针的性能直接影响扫描探针显微镜的分辨率和拓扑成像质量,因此玻璃微探针的制备在实现纳米生物样品的高分辨率探测中具有重要的意义。文章运用扫描电子显微镜直接观测玻璃微探针的几何形状进而推算电阻值,借助膜片钳技术测量微探针实际电阻,并通过应用该种玻璃探针对标准样品进行扫描成像来判断该微探针是否满足实验需要,最终综合上述数据对纳米尺度玻璃微探针的性能进行评价。
Scanning Ion Conductance Microscopy is a scanning probe microscope technology recently developed in the nanomedicine field that can be used to study the surface morphology of living cells under high-resolution, non-contact conditions under physiological conditions. The performance of the glass microprobe for scanning directly affects the resolution and topological imaging quality of the scanning probe microscope. Therefore, preparation of the glass microprobe is of great significance for realizing high-resolution detection of the nano-biological sample. The article uses the scanning electron microscope to directly observe the geometry of the glass microprobe and then deduces the resistance value, and uses the patch clamp technique to measure the actual resistance of the microprobe. By using the glass probe to scan the standard sample to judge the microprobe Whether the needles meet the experimental needs, and finally the above data to evaluate the performance of nanoscale glass microprobe.