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目的探讨阿藿烯对四氯化碳(CCl4)所致大鼠肝纤维化的保护作用。方法将40只SD大鼠随机分为对照组、模型组、低(25mg/kg)和高剂量(50mg/kg)阿藿烯组,用40%CCl4皮下注射制作肝纤维化模型,阿藿烯按低和高剂量分别连续灌胃6w后,检测血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性、总蛋白(TP)和清蛋白(ALB)含量、总胆红素(TBIL)和间接胆红素(DBIL)及肝纤维化的透明质酸(HA)和层粘连蛋白(LN)水平;检测肝组织超氧化物歧化酶(SOD)活性和脂质过氧化(MDA)水平;并进行肝脏组织病理学检查。结果低、高剂量组阿藿烯均可显著降低血清ALT和AST活性、TBIL、DBIL、HA和LN水平(P<0.01或P<0.05);增加ALB含量(P<0.05);提高肝脏SOD活性和减少MDA含量(P<0.01);并明显改善肝脏的纤维化程度(P<0.01或P<0.05)。结论阿藿烯能够减轻CCl4所致的大鼠肝纤维化作用,其机制可能与抗氧化作用有关。
Objective To investigate the protective effect of alloxan on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats. Methods Forty Sprague-Dawley rats were randomly divided into control group, model group, low (25mg / kg) and high dose (50mg / kg) methotrexate groups. Subcutaneous injection of 40% The serum ALT, AST, total protein (TP) and albumin (ALB) were measured after 6 and 6 weeks of continuous gavage. Bilirubin (TBIL), indirect bilirubin (DBIL) and hyaluronic acid (HA) and laminin (LN) in hepatic fibrosis were detected. The activity of superoxide dismutase (SOD) Oxidation (MDA) levels; and liver histopathology. RESULTS: Alloxene significantly decreased serum ALT and AST activity, TBIL, DBIL, HA and LN levels (P <0.01 or P <0.05), increased ALB content (P <0.05) And decreased MDA content (P <0.01), and significantly improved the liver fibrosis (P <0.01 or P <0.05). Conclusions Alloxene can reduce the hepatic fibrosis induced by CCl4 in rats, which may be related to the anti-oxidation effect.