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目的:探讨血管内皮生长因子受体-1(Flt-1)、血小板衍生生长因子受体(PDGFR)、碱性成纤维生长因子受体(bFGFR)及微血管密度(MVD)的表达及相关性研究。方法:分别选择71例手术切除的胃癌及39例正常胃组织石蜡标本为观察组及对照组,应用免疫组织化学方法检测组织中Flt-1、PDGFR和bFGFR的蛋白表达及MVD。结果:Flt-1、PDGFR和bFGFR在胃癌组织中的阳性率分别为53.5%、49.3%和50.7%,高于对照组(12.8%、10.3%、7.7%),χ2值分别为17.514、16.765和20.350,P值均为0.000。且上述受体的表达与胃癌的TNM分期、浸润及淋巴结、远处转移密切相关,P<0.01或P<0.05。胃癌组MVD(45.2±15.2)高于对照组(28.6±4.2),t=5.083,P=0.001。MVD与肿瘤的TNM分期、浸润深度、淋巴结和远处转移密切相关,P<0.01。3种受体在胃癌中的表达存在正相关,P<0.01。3种受体在胃癌组织中表达均阳性伴有淋巴结转移的发生率为91.7%,高于三者表达均阴性者(68.2%),χ2=4.023,P=0.045;向远处转移的发生率分别为58.3%和18.2%,χ2=7.769,P=0.005。三者表达均阳性和均阴性的胃癌组织中MVD计数分别为60.5±18.0和40.4±5.1,两组比较差异有统计学意义,t=21.38,P=0.001。结论:3种受体在胃癌组织中表达增高,且与MVD有相关性,它们在胃癌的发生、发展、浸润及转移中可能存在相互诱导及协同作用。
Objective: To investigate the expression and correlation of Flt-1, PDGFR, bFGFR and MVD . Methods: Seventy-one paraffin-embedded specimens of gastric cancer and 39 normal gastric tissues were selected as observation group and control group respectively. Immunohistochemistry was used to detect the protein expression and MVD of Flt-1, PDGFR and bFGFR. Results: The positive rates of Flt-1, PDGFR and bFGFR in gastric cancer were 53.5%, 49.3% and 50.7%, respectively, which were higher than those in the control group (12.8%, 10.3%, 7.7% 20.350, P values are 0.000. The expressions of these receptors were closely related to the TNM stage, invasion, lymph node metastasis and distant metastasis of gastric cancer, P <0.01 or P <0.05. MVD in gastric cancer group (45.2 ± 15.2) was higher than that in control group (28.6 ± 4.2), t = 5.083, P = 0.001. MVD was closely related to tumor TNM stage, depth of invasion, lymph node and distant metastasis, P <0.01.3 There was a positive correlation between MVD and gastric cancer (P <0.01.3) The incidence of lymph node metastasis was 91.7%, which was higher than that of the three cases (68.2%), χ2 = 4.023, P = 0.045. The incidence of distant metastasis was 58.3% and 18.2% respectively, χ2 = 7.769 , P = 0.005. The MVD counts of gastric cancer tissues with positive expression and negative expression were 60.5 ± 18.0 and 40.4 ± 5.1, respectively, with significant difference between the two groups (t = 21.38, P = 0.001). CONCLUSION: The three receptors are highly expressed in gastric cancer and have a correlation with MVD. They may induce and synergize with each other in the occurrence, development, infiltration and metastasis of gastric cancer.