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为在实验室中全面评价葡萄球菌的致病性,作者研究了金黄色葡萄球菌(金葡菌)和表皮葡萄球菌(表葡菌)对免血及兔血清杀菌作用的抗性。对血液杀菌作用抗性的测定:将24小时葡萄球菌培养物制成一定浓度的菌悬液,用内径1mm的接种环取一环菌悬液与3滴兔血、2滴2%柠檬酸钠溶液混匀。先移一环到肉膏蛋白胨琼脂平板表面并抹匀。混悬液于37℃孵育60及120分钟后,再按上法涂抹第2、第3块平板,孵育18～20小时后计算菌落总数,与原菌数(即第一块琼脂平板上菌数)比
In order to fully evaluate the pathogenicity of staphylococci in the laboratory, the authors studied the resistance of Staphylococcus aureus and Staphylococcus epidermidis (Staphylococcus epidermidis) against blood and rabbit serum bactericidal activity. Determination of resistance to blood bactericidal effect: Staphylococcus aureus culture for 24 hours to a certain concentration of bacterial suspension, with an inner diameter of 1mm loop ring bacteria take a ring of bacteria and 3 drops of rabbit blood, 2 drops of 2% sodium citrate Mix the solution. First move a ring to the meat peptone agar plate surface and wipe. After the suspension was incubated at 37 ° C for 60 and 120 minutes, the second and third plates were smeared again according to the above method. The total number of colonies was counted after incubation for 18 to 20 hours, )ratio