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目的观察重组人促红细胞生成素(rhEPO)对新型支气管肺发育不良(BPD)早产鼠模型肺组织血管内皮生长因子(VEGF)蛋白及mRNA表达的影响。方法60只定期受孕SD大鼠于孕第15天孕囊内注射脂多糖(LPS)或磷酸盐缓冲液(PBS),孕第21天剖宫娩出早产鼠,早产鼠于出生后6h内分为5组:PBS+空气、PBS+高氧、LPS+高氧、PBS+高氧+rhEPO、LPS+高氧+rhEPO组。rhEPO(1200 IU/kg)干预组于高氧暴露6h后开始第1次背部皮下注射,隔天1次,共7次。分别在高氧暴露第1、7、14天处死早产鼠,观察各组生存率、体质量、肺质量并计算肺质量/体质量、肺组织病理变化,采用Westernblot和RT-PCR法检测肺组织VEGF蛋白及mRNA表达水平。结果 与PBS+空气组比较,经高氧干预后生存率降低、体质量减轻、肺质量增加,病理损伤加重,肺组织VEGF蛋白及mRNA表达量降低,并且以LPS+高氧组更显著(P<0.05或P<0.01),肺质量/体质量有增加趋势(P>0.05);rhEPO干预后与相应对照组比较上述指标得到相应改善(P<0.05或<0.01)。结论 rhEPO可能通过参与调解VEGF相关通路,对BPD模型早产鼠起到一定的干预和治疗作用,提高生存率。
Objective To investigate the effects of recombinant human erythropoietin (rhEPO) on the expression of vascular endothelial growth factor (VEGF) and mRNA in the lung tissue of a new model of premature lung failure in children with bronchopulmonary dysplasia (BPD). Methods Sixty pregnant SD rats were injected intraperitoneally with lipopolysaccharide (LPS) or phosphate buffered saline (PBS) on day 15 of pregnancy. Premature rats were delivered by cesarean section on the 21st day of pregnancy. The premature rats were divided into 6 h after birth 5 groups: PBS + air, PBS + hyperoxia, LPS + hyperoxia, PBS + hyperoxia + rhEPO, LPS + hyperoxia + rhEPO group. In the rhEPO (1200 IU / kg) intervention group, the first subcutaneous injection on the back began 6 hours after hyperoxia exposure and was given once a day for 7 times. The rats were sacrificed on the 1st, 7th and 14th day after hyperoxia exposure respectively. The survival rate, body weight, lung mass and lung mass / body weight and pathological changes of the lungs were observed. The lung tissues were detected by Western blot and RT-PCR VEGF protein and mRNA expression levels. Results Compared with the PBS + air group, the survival rate decreased, the body weight decreased, the lung mass increased, the pathological injury increased, and the expression of VEGF protein and mRNA in the lung tissue decreased after LPS plus hyperoxia group (P <0.05 (P & gt; 0.05 or & lt; 0.01), P <0.01). The lung mass / body weight had an increasing trend (P> 0.05). Conclusions rhEPO may play a certain role in intervention and treatment of premature rats by BPD model by participating in the regulation of VEGF related pathways, and improve the survival rate.