论文部分内容阅读
目的:探讨孕期酒精暴露对仔鼠海马神经细胞自噬的影响。方法:建立孕期酒精暴露动物模型,采用透射电子显微镜、免疫荧光和Western Blot技术观察P0、P7和P14仔鼠海马CA1区锥体细胞自噬情况。结果:免疫荧光结果显示,同一时间点对照组仔鼠LC3阳性率明显少于酒精低剂量组和高剂量组,差异有统计学意义(P<0.05),且随着酒精剂量的增加自噬作用增强;同时,随着年龄的增加,各年龄组间LC3表达的阳性率下降(P<0.05)。电镜下酒精组锥体细胞内出现较多自噬体或自噬溶酶体结构,细胞器损伤严重。Western Blot检测各组间CA1区锥体细胞LC3蛋白的相对表达量与上述结果一致。结论:自噬参与了仔鼠海马CA1区锥体细胞的正常发育,在一定时期内随着生长发育,自噬作用逐渐减弱;孕期酒精暴露能导致仔鼠海马CA1区锥体细胞自噬增强,且有剂量依赖性和长时程效应。
Objective: To investigate the effect of prenatal alcohol exposure on autophagy in hippocampal neurons of offspring. Methods: Animal model of alcohol exposure during pregnancy was established. The autophagy of hippocampal CA1 pyramidal neurons of P0, P7 and P14 pups were observed by transmission electron microscopy, immunofluorescence and Western Blot. Results: The results of immunofluorescence showed that the positive rate of LC3 in the pups of the control group was significantly less than that of the low dose alcohol group and the high dose group at the same time point (P <0.05), and the autophagy was increased with the increase of the alcohol dose While the positive rate of LC3 expression among all age groups decreased with the increase of age (P <0.05). Under electron microscope, more autophagosomes or autophagic lysosomes appeared in the pyramidal cells of alcohol group, and the organelles were damaged seriously. Western Blot detection of CA1 pyramidal cells in each group LC3 protein relative expression level with the above results. Conclusions: Autophagy is involved in the normal development of pyramidal cells in hippocampal CA1 region of offspring rats, and gradually decreases with the growth and development of autophagy in the hippocampal CA1 region of the offspring. Exposure to alcohol during pregnancy can lead to increased autophagy in hippocampal CA1 pyramidal cells, And a dose-dependent and long-term effects.