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目的探讨Toll样受体4(toll-like receptor 4,TLR4)信号活化对Peyer氏结(peyer’s patches,PPs)来源的树突状细胞(PP-derived dendritic cells,PPDCs)功能的影响,并与脾脏来源的树突状细胞(spleen-derived dendritic cells,SPDCs)进行比较。方法脂多糖(lipopolysaccharide,LPS)刺激小鼠SPDCs和PPDCs,ELISA法检测培养上清中细胞因子表达;将LPS刺激的树突状细胞(dendritic cells,DCs)与CD4+初始T细胞共培养,流式细胞仪检测分泌干扰素γ(interferon-γ,IFN-γ)、白介素(interleukin,IL)-17和IL-10的CD4+T细胞的频数。结果 LPS促进SPDCs分泌IL-6,IL-10,IL-12和TNF-α(均有P<0.05),但仅明显提高PPDCs产生IL-6和IL-10的能力(均有P<0.05),而不影响IL-12和TNF-α的产生(均有P>0.05);LPS刺激后,SPDCs能够诱导CD4+初始T细胞分化为分泌IFN-γ和IL-10的CD4+T细胞(均有P<0.05),而PPDCs则诱导其分化为分泌IL-10的CD4+T细胞(t=5.63,P=0.005)。结论 TLR4受体在PPDCs和SPDCs中发挥着不同的调节作用。
Objective To investigate the effect of toll-like receptor 4 (TLR4) signaling activation on the function of PP-derived dendritic cells (PPDCs) in Peyer’s patches (PPs) Derived spleen-derived dendritic cells (SPDCs) were compared. Methods SPDCs and PPDCs were stimulated with lipopolysaccharide (LPS) and cytokines in culture supernatants were detected by ELISA. Dendritic cells (DCs) stimulated with LPS were cocultured with CD4 + naive T cells and the flow cytometry Cytometry was used to detect the frequency of CD4 + T cells secreting interferon-γ (IFN-γ), interleukin (IL) -17 and IL-10. Results LPS promoted the secretion of IL-6, IL-10, IL-12 and TNF-α by SPDCs (all P <0.05), but only increased the production of IL-6 and IL-10 by PPDCs (all P < (All P> 0.05). After LPS stimulation, SPDCs could induce the differentiation of CD4 + naive T cells into CD4 + T cells secreting IFN-γ and IL-10 P <0.05), while PPDCs induced their differentiation into CD4 + T cells secreting IL-10 (t = 5.63, P = 0.005). Conclusion TLR4 receptors play different regulatory roles in PPDCs and SPDCs.