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细胞膜钙离子(Ca~(2+))转运障碍与高血压发病有密切关系。作者最近建立了红细胞外翻囊泡(inside-out vesicle简称IOV)钙摄取的方法,并为研究高血压时细胞膜Ca~(2+)摄取异常机理提供了一个有力手段。红细胞首先用低渗缓冲液(10mmol/LTris-HCl,pH7.6～7.8)破膜,并重复三次直至得到去血红蛋白的红细胞影泡(RBC-Ghost,呈乳白色)。然后将其置于低渗、碱性环境内(0.5mmol/LTris-Hcl+50μmolβ-mercaptoethanol+20μmol/LTris-EDTA,ph8.5)保温,以促使膜经入胞作用(endo-
Cell membrane calcium (Ca2 +) transport disorder and the incidence of hypertension are closely related. The authors recently established a method of calcium uptake of the red blood cell outside of the vesicle (IOV) and provided a powerful tool for studying the abnormal Ca ~ (2+) uptake mechanism in hypertensive patients. Erythrocytes were first ruptured with hypotonic buffer (10 mmol / L Tris-HCl, pH 7.6-7.8) and repeated three times until a hemoglobin-depleted red blood cell vesicle (RBC-Ghost, milky white) was obtained. The cells were then incubated in a hypotonic, alkaline environment (0.5 mmol / L Tris-Hcl + 50 μmol β-mercaptoethanol + 20 μmol / L Tris-EDTA, pH 8.5)