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G类有机磷毒剂在水分子的参予下可以自发地水解,1摩尔毒剂生成1摩尔有机酸和1摩尔无机酸。G类毒剂水解酶是一类族专一性生物催化剂,可以大大加速上述反应。根据催化理论,酶可以同时催化正向及逆向两种反应的进行。本实验观察了G类毒剂水解酶存在条件下,梭曼水解产物在试管内合成梭曼的可能性及程度。这一合成的证实在理论上及梭曼中毒防治中都有一定的价值。材料与方法一、G类毒剂水解酶制品家兔经颈动脉放血,每ml全血加0.1mlACD溶液或10％柠檬酸钠抗凝。离心取血浆,-20℃冻结后在室温融化,如此反复3次,以破坏可能存在的V类毒剂氧化酶。而
Class G organic phosphate poisons can spontaneously hydrolyze with the participation of water molecules, and 1 mole of poison generates 1 mole of organic acid and 1 mole of inorganic acid. G-class toxic hydrolases are a family of class-specific biological catalysts that greatly accelerate the above reaction. According to the catalytic theory, the enzyme can catalyze both forward and reverse reactions. In this experiment, we observed the possibility and degree of soman hydrolysates in somatic tube synthesis in the presence of G-type toxic hydrolase. This synthesis confirmed in theory and soman poisoning have a certain value. Materials and methods A, G class drug hydrolase products rabbit carotid artery bleeding, 0.1 mlACD solution per ml of whole blood or 10% sodium citrate anticoagulation. Plasma was centrifuged and thawed at -20 ° C and then thawed at room temperature. This was repeated 3 times to destroy any V-type oxidase enzymes that may be present. and