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目的了解成都市2010-2013年肠道病毒71型VP1基因变异情况,为疾病防控提供依据。方法选取2010-2013年(上)荧光PCR检测为EV71阳性标本,扩增测序其VP1全长,构建进化树并进行相关生物信息学分析。结果 25份标本基因亚型均为C4亚型,与阜阳株差异不大,核苷酸同源性82.72%~100%,氨基酸同源性为96.29%~100%,氨基酸变异位点11个。结论成都市2010-2013年(上)肠道病毒71型变异不明显,但需关注关键氨基酸突变位点及其可能带来的防控挑战。
Objective To understand the variation of VP1 gene of enterovirus type 71 from 2010 to 2013 in Chengdu and to provide basis for disease prevention and control. Methods From 2010 to 2013 (up), fluorescent PCR was used to detect EV71 positive samples. The full length of VP1 was sequenced and the phylogenetic tree was constructed and analyzed by bioinformatics analysis. Results The 25 subtypes were all subtypes of C4 subtype, with no significant difference from Fuyang strain. The nucleotide homology was 82.72% ~ 100%, the amino acid homology was 96.29% ~ 100%, and the amino acid variation was 11. Conclusion The variation of enterovirus 71 in 2010-2013 was not obvious in Chengdu, but attention should be paid to the key amino acid mutation sites and the possible prevention and control challenges.