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目的:探讨芒果苷的抗炎作用机制。方法:脂多糖(LPS)+烟雾诱导法建立大鼠慢性支气管炎模型,测定肺泡灌洗液(BALF)和血清中的超氧化岐化酶(SOD)活性、丙二醛(MDA)、一氧化氮(NO)含量,肺组织中肿瘤坏死因子(TNF-α)、白细胞介素8(IL-8)含量;逆转录聚合酶链反应(RT-PCR)检测LPS诱导RAW264.7小鼠巨噬细胞环氧化酶-2(COX-2)mRNA的表达。结果:灌胃芒果苷高、中、低剂量(400,200,100 mg.kg-1)均使慢性支气管炎大鼠BALF和血清中的SOD活性、NO含量明显升高,MDA含量明显降低;肺组织中TNF-α,IL-8含量明显降低。200,100,50μmol.L-1芒果苷均可显著降低LPS诱导下的RAW264.7 COX-2 mRNA表达。结论:芒果苷抗炎机制可能与升高SOD活性,NO含量,降低MDA含量,下调TNF-α,IL-8,COX-2 mRNA的表达,减轻炎症反应有关。
Objective: To investigate the anti-inflammatory mechanism of mangiferin. Methods: Chronic bronchitis model was induced by lipopolysaccharide (LPS) + aerosol in rats. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and alveolar lavage fluid (BALF) (NO), tumor necrosis factor (TNF-α) and interleukin-8 (IL-8) in lung tissue were detected by reverse transcription-polymerase chain reaction Cell cyclooxygenase-2 (COX-2) mRNA expression. Results: The activities of SOD and the content of NO in BALF and serum of rats with chronic bronchitis were significantly increased at high, medium and low doses (400, 200 and 100 mg.kg-1), respectively. The contents of NO and MDA in lung tissue were significantly decreased -α, IL-8 content was significantly reduced. 200, 100, 50μmol.L-1 mangiferin significantly reduced the LPS-induced RAW264.7 COX-2 mRNA expression. CONCLUSION: The anti-inflammatory mechanism of mangiferin may be related to increasing SOD activity, NO content, decreasing MDA content, decreasing the expression of TNF-α, IL-8 and COX-2 mRNA and reducing the inflammatory reaction.